Horseradish Peroxidase Functionalized Fluorescent Gold Nanoclusters for Hydrogen Peroxide Sensing
Citations Over TimeTop 1% of 2011 papers
Abstract
The fluorescence of metal nanoclusters provides an amusing optic feature to be applied in various fields. However, rational design of dual functional fluorescent metal nanoclusters directed by active enzyme with targeted application remains little explored. In this work, we report a new strategy to construct enzyme functionalized fluorescent gold nanoclusters via a biomineralization process for the detection of hydrogen peroxide. Horseradish peroxidase (HRP) was used as a model functional template to direct the synthesis of fluorescent gold nanoclusters (Au NCs) at physiological conditions to form HRP-Au NCs bioconjugates. We found that the fluorescence of HRP-Au NCs can be quenched quantitatively by adding H(2)O(2), indicating that HRP enzyme remains active and enables catalytic reaction of HRP-Au NCs and H(2)O(2). Upon the addition of H(2)O(2) under optimal conditions, the fluorescence intensity quenched linearly over the range of 100 nM to 100 μM with high sensitivity (LOD = 30 nM, S/N = 3). This study would be potentially extended to other functional proteins to generate dual functional nanoclusters and applied to real time monitoring of biologically important targets in living cells.
Related Papers
- → Inactivation of horseradish peroxidase by phenol and hydrogen peroxide: a kinetic investigation(1994)173 cited
- → Ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase(2010)14 cited
- → New fluorogenic substrates for horseradish peroxidase: Rapid and sensitive assays for hydrogen peroxide and the peroxidase(1980)195 cited
- → Luminol chemiluminescence reaction catalyzed by a microbial peroxidase(1990)73 cited
- → Evaluation of Textile Dye Degradation Due to the Combined Action of Enzyme Horseradish Peroxidase and Hydrogen Peroxide(2014)17 cited