Modified Enzyme-Linked Immunosorbent Assay Strategy Using Graphene Oxide Sheets and Gold Nanoparticles Functionalized with Different Antibody Types
Analytical Chemistry2013Vol. 85(13), pp. 6228–6232
Citations Over TimeTop 10% of 2013 papers
Hongjun Lin, Yingfu Liu, Jingrui Huo, Aihong Zhang, Yiting Pan, Haihong Bai, Jiao Zhang, Fang Tian, Xin Wang, Yun Cai, Qingming Wang, Yangjun Zhang, Xiaohong Qian
Abstract
Gold nanoparticles (GNPs) and graphene oxide (GO) sheets are excellent nano carriers in many analytical methods. In this study, a modified enzyme-linked immunosorbent assay (ELISA) strategy was developed using antibody-functionalized GO sheets and GNPs. This modification significantly reduced the limit of detection (LOD) and cost greatly of this assay. The applicability of the method was demonstrated by detecting HSP70 in a human serum sample. This result suggests that the 3G-ELISA method is feasible to detect an antigen in a complex mixture, and the LOD is up to 64-fold and the cost is as low as one-tenth of the conventional ELISA method.
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