Aptamer-Based Affinity Chromatographic Assays for Thrombin

Citations Over TimeTop 11% of 2008 papers

Abstract

Affinity chromatographic assays for thrombin were developed using two aptamers as affinity ligands. The efficient capture and step elution of thrombin with NaClO4 enabled the determination of thrombin by using either absorbance or fluorescence detection. Preconcentration of thrombin on the affinity column improved the detection limit of thrombin to 0.1 nM. Using an aptamer for the fibrinogen-binding site of thrombin and a second aptamer for the heparin-binding site, a sandwich chromatographic assay was developed, showing improved selectivity of thrombin detection and eliminating the need for labeling thrombin in the sample. The increased local concentration of aptamers immobilized on monolithic columns favored the formation of aptamer-thrombin complexes, resulting in improved retention and detection of thrombin at trace levels.

Related Papers

• → Aptamer cocktails: Enhancement of sensing signals compared to single use of aptamers for detection of bacteria(2013)87 cited
• → An aptamer cocktail-based electrochemical aptasensor for direct capture and rapid detection of tetracycline in honey(2019)40 cited
• → Idiosyncrasies of Thermofluorimetric Aptamer Binding Assays(2019)10 cited
• → Selecting DNA aptamers for endotoxin separation(2015)5 cited
• → Aptameric sensors based on structural change for diagnosis(2010)9 cited