Electrochemical DNA Biosensor Based on the Proximity-Dependent Surface Hybridization Assay

Citations Over TimeTop 10% of 2009 papers

Abstract

This paper describes a novel electrochemical DNA (E-DNA) biosensor for simple, rapid, and specific detection of nucleic acids based on the proximity-dependent surface hybridization assay. This E-DNA biosensor was constructed by self-assembly of a 3' short thiolated capture probe on the gold electrode. DNA detection was realized by outputting a remarkable redox current of the 5' ferrocene (Fc) tail labeled probe. When the target DNA was introduced into the system, it was complementary to the 5' Fc labeled probe at the one-half-segment and complementary to the 3' short thiolated capture probe at the other half-segment, resulting in forming a stable duplex complex. As a result, the Fc probe was proximate to the electrode surface, and the Faradaic current was observed. This E-DNA biosensor was proved to have a low detection limit (1 fM) and a wide dynamic range (from 1 fM to 1 nM) due to the stable hybridization mode. In addition, the sensing system could discriminate the complementary sequence from mismatch sequences, with high sensitivity, stability, and reusability.

Related Papers

• → Peptide nucleic acid pre-gel hybridization: An alternative to Southern hybridization(1996)74 cited
• → Affinity Capture and Recovery of DNA at Femtomolar Concentrations with Peptide Nucleic Acid Probes(2000)26 cited
• → Hybridization Study of PNA-DNA in the Solution and Surface-Solution Interface for Biosensor Application(2009)3 cited
• → Peptide nucleic acid (PNA) biosensors for DNA recognition(2001)1 cited
• A Systemic Comparison between PNA Probe and DNA Probe(2006)