Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells
Analytical Chemistry2010Vol. 82(6), pp. 2192–2203
Citations Over TimeTop 1% of 2010 papers
Abstract
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.
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