Capillary Electrophoresis of Supercoiled and Linear DNA in Dilute Hydroxyethyl Cellulose Solution
Analytical Chemistry1997Vol. 69(6), pp. 1192–1196
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Richard W. Hammond, Hidehiro Oana, Jeffrey J. Schwinefus, Jeffrey Bonadio, Robert J. Levy, Michael D. Morris
Abstract
Capillary electrophoresis in dilute hydroxyethyl cellulose is shown to separate supercoiled DNA in the size range 2000-16,000 base pairs. The plasmids migrate more slowly than linear ds-DNA of the same sizes. Plasmid bandwidths are larger than observed for ds-DNA, allowing identification of the type of DNA by bandwidth. The differing dependence of mobility on chain length can be explained by assuming that a plasmid migrates as an elastic rod, while ds-DNA migrates as a wormlike chain.
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