High-Speed Separation of Linear and Supercoiled DNA by Capillary Electrophoresis. Buffer, Entangling Polymer, and Electric Field Effects
Analytical Chemistry1998Vol. 70(3), pp. 574–579
Citations Over TimeTop 10% of 1998 papers
Hidehiro Oana, Richard W. Hammond, Jeffrey J. Schwinefus, Shau-Chun Wang, Masao Doi, Michael D. Morris
Abstract
Capillary electrophoresis in dilute and semidilute (slightly entangled) hydroxyethyl cellulose (HEC) is shown to separate linear double-stranded DNA (ds-DNA) and supercoiled plasmid DNA in the size range 1-16 thousand base pairs in 3 min. The mobilities of linear ds-DNA fragments are stronger functions of electric field strength and buffer concentration than the mobilities of supercoiled plasmids. The effects of HEC concentration and molecular weight are similar for both forms of DNA. The behavioral differences, which are attributed to the greater stiffness of the plasmids, can be used to define conditions that maximize resolution of supercoiled and linear ds-DNA of the same or similar number of base pairs.
Related Papers
- → Capillary electrophoresis of DNA in uncrosslinked polymer solutions: Evidence for a new mechanism of DNA separation(2000)29 cited
- → Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis(2003)13 cited
- → Separation of Double- and Single-Stranded DNA Restriction Fragments: Capillary Electrophoresis with Polymer Solutions under Alkaline Conditions(1999)37 cited
- → The emergence of sequence-dependent structural motifs in stretched, torsionally constrained DNA(2019)
- → Chloroquine agarose gel v1(2018)