Structural Characterization of Carbohydrate Sequence, Linkage, and Branching in a Quadrupole Ion Trap Mass Spectrometer: Neutral Oligosaccharides and N-Linked Glycans

Citations Over TimeTop 10% of 1998 papers

Abstract

Several oligosaccharide and N-linked glycan samples have been utilized to evaluate structural detail obtained with an ion trap mass spectrometer (ITMS). Using multistage MS/MS (MSn) in a commercial instrument, linear sequence, linkage, and branching have been studied, as well as the positional isomers within branched glycans. Samples were prepared as methylated derivatives, which amplifies the structurally informative cross-ring and double glycosidic cleavage products associated with interresidue linkage and oligomer branching detail. Spectral analysis of a linear glucose homopolymer demonstrated ITMS to be comparable with data obtained from a triple-quadrupole instrument, and in a study of complex glycans, the feature of MSn enabled the assignment of linkages that cannot be assessed by conventional triple-quadrupole tandem instrumentation. The longer time scale of CID experiments enhances the abundance of multiple bond ruptures important for a complete understanding of structure. This technology appears to provide a significant step toward the goal of characterizing all aspects of carbohydrate structure using a single instrument.

Related Papers

• → Triple quadrupole linear ion trap mass spectrometer for the analysis of small molecules and macromolecules(2004)284 cited
• → Rapid screening and characterization of drug metabolites using a new quadrupole–linear ion trap mass spectrometer(2003)128 cited
• → Use of a quadrupole linear ion trap mass spectrometer in metabolite identification and bioanalysis(2003)118 cited
• → Rapid screening and characterization of drug metabolites using a multiple ion monitoring–dependent MS/MS acquisition method on a hybrid triple quadrupole‐linear ion trap mass spectrometer(2008)89 cited
• → High-performance liquid chromatography–tandem mass spectrometry with a new quadrupole/linear ion trap instrument(2003)87 cited