Poly(ethylene glycol) Hydrogel-Encapsulated Fluorophore−Enzyme Conjugates for Direct Detection of Organophosphorus Neurotoxins
Analytical Chemistry1999Vol. 71(21), pp. 4909–4912
Citations Over TimeTop 13% of 1999 papers
Abstract
A simple approach is described for preparing poly-(ethylene glycol) hydrogel materials with encapsulated seminapthofluorescein (SNAFL)-organophosphorus hydrolase enzyme conjugates. Direct determination of enzyme-catalyzed neurotoxin hydrolysis is provided by the self-referencing, pH-sensitive dye SNAFL-1, whose emission spectrum changes at lambda = 550 in response to pH. Using spectrofluorimetry and paraoxon as a model organophosphate, paraoxon concentrations as low as 8 x 10(-7) M could be readily detected. On the basis of the signal-to-noise ratio, a detection limit of 16 nM was determined. The materials demonstrated high stability against enzyme-denaturing, leaching, and photobleaching when stored under ambient conditions.
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