Characterization of Cellular Optoporation with Distance
Citations Over TimeTop 10% of 2000 papers
Abstract
We have developed and characterized cellular optoporation with visible wavelengths of light using standard uncoated glass cover slips as the absorptive media. A frequency-doubled Nd:YAG laser pulse was focused at the interface of the glass surface and aqueous buffer, creating a stress wave and transiently permeabilizing nearby cells. Following optoporation of adherent cells, three spatial zones were present which were distinguished by the viability of the cells and the loading efficiency (or number of extracellular molecules loaded). The loading efficiency also depended on the concentration of the extracellular molecules and the molecular weight of the molecules. In the zone farthest from the laser beam (> 60 microns under these conditions), nearly all cells were both successfully loaded and viable. To illustrate the wider applicability of this optoporation method, cells were loaded with a substrate for protein kinase C and the cellular contents then analyzed by capillary electrophoresis. In contrast to peptides loaded by microinjection, optoporated peptide showed little proteolytic degradation, suggesting that the cells were minimally perturbed. Also demonstrating the potential for future work, cells were optoporated and loaded with a fluorophore in the enclosed channels of microfluidic devices.
Related Papers
- → Design of Sub-40nm FinFET Based Label Free Biosensor(2022)16 cited
- → Reconstruction of Fluorophore Concentration Variation in Dynamic Fluorescence Molecular Tomography(2014)15 cited
- → Design and application of near-infrared fluorophore based on a novel thiazolidinedione-functionalized dicyanoisophorone(2020)14 cited
- → Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding(2006)14 cited
- THE PHOTOCHEMICAL METHODS OF IMMOBILIZATION BIOMOLECULES ON POLYMER MATERIALS(2001)