UV Resonance Raman Detection and Quantitation of Domoic Acid in Phytoplankton
Citations Over TimeTop 24% of 2000 papers
Abstract
Cultures of the phytoplankton diatom, Pseudonitzschia multiseries, have been harvested under controlled growth conditions ranging from late logarithmic to late stationary phase (17-58 days). The amount of domoic acid (DA) present in the growth media and in the homogenized cells has been determined by HPLC. Defined samples of media, homogenized cells, whole cells, and whole cells in media have been laser excited at 251 nm for the purpose of selectively exciting intense UV resonance Raman spectra from DA in the samples. Neither media nor cell component spectra from algae seriously interfere with DA spectra. The spectral cross sections for the dominant 1652-cm-1 mode of DA have been determined for 242-, 251-, and 257-nm excitation. Maximum sensitivities are achieved with 251-nm excitation because cross sections for DA are a maximum, and interference from other algal components becomes very small. DA concentrations that have been determined with 251-nm excitation by resonance Raman methods correlate closely with values determined independently with HPLC, especially at higher DA concentrations. The UV resonance Raman analysis of DA in phytoplankton algae is shown to be very sensitive and quantitative as well as rapid and nonintrusive.
Related Papers
- → Novel symptomatology and changing epidemiology of domoic acid toxicosis in California sea lions ( Zalophus californianus ): an increasing risk to marine mammal health(2007)204 cited
- → Comparative toxicity to mice of domoic acid and isodomoic acids A, B and C(2008)43 cited
- → Occurrence of the toxic diatom Nitzschia pungens f. multiseries in Massachusetts Bay, Massachusetts, USA(1994)31 cited
- → Validation of the Analysis of Domoic Acid using High Performance Liquid Chromatography(2010)2 cited