Integration of an Immunosorbent Assay System: Analysis of Secretory Human Immunoglobulin A on Polystyrene Beads in a Microchip
Analytical Chemistry2000Vol. 72(6), pp. 1144–1147
Citations Over TimeTop 1% of 2000 papers
Kiichi Sato, Manabu Tokeshi, Tamao Odake, Hiroko Kimura, Takeshi Ooi, Masayuki Nakao, Takehiko Kitamori
Abstract
An immunosorbent assay system was integrated into a glass microchip. Polystyrene beads were introduced into a microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reacted with colloidal gold conjugated anti-s-IgA antibody and detected by a thermal lens microscope. The scale merits of liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integration cut the time necessary for the antigen-antibody reaction by 1/90, thus shortening the overall analysis time from 24 h to less than 1 h. Moreover, troublesome operations required for conventional immunosorbent assays could be replaced by simple operations.
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