Identification and Quantification of Flavonoids in Human Urine Samples by Column-Switching Liquid Chromatography Coupled to Atmospheric Pressure Chemical Ionization Mass Spectrometry
Citations Over TimeTop 10% of 2000 papers
Abstract
A rapid and sensitive high-performance liquid chromatographic mass spectrometric (HPLC-MS) method is described for the determination and quantification of 12 dietary flavonoid glycosides and aglycons in human urine samples. Chromatographic separation of the analytes of interest was achieved by column-switching, using the first column (a Zorbax 300SB C-3 column) for sample cleanup and eluting the heart-cut flavonoid fraction onto the second column (a Zorbax SB C-18 column) for separation and detection by ultraviolet and atmospheric pressure chemical ionization MS using single ion monitoring in negative mode. The fragmentor voltage was optimized with regard to maximum abundance of the molecular ion and qualifier ions of the analytes. Calibration graphs were prepared for urine, and good linearity was achieved over a dynamic range of 2.5-1000 ng/mL. The inter- and intraassay coefficients of variation for the analysis of the 12 different flavonoids in quality control urine samples were 12.3% on average (range 11.0-13.7%, n = 24, reproducibility) and the repeatability of the assay were 5.0% (mean, range 0.1-14.8%, n = 12). A subset of 10 urine samples from a human dietary intervention study with high and low flavonoid content was analyzed, and the results are reported.
Related Papers
- → Qualitative and Quantitative Analyses of Phenolic Compounds by High-performance Liquid Chromatography and Detection with Atmospheric Pressure Chemical Ionization Mass Spectrometry(1996)25 cited
- → Atmospheric Pressure Chemical Ionization Mass Spectrometry(1985)15 cited
- → [13] Atmospheric pressure chemical ionization and electron capture negative chemical ionization mass spectrometry in studying β-carotene conversion to retinol in humans(1997)27 cited
- → Liquid chromatography–mass spectrometric analysis of phenylurea herbicides and their degradation products using an atmospheric pressure ionization interface(1998)8 cited
- Dual Chemical Ionization Inlet for fast switching of reagent ion chemistry in atmospheric pressure chemical ionization mass spectrometry applications(2018)