Zeptomole-Sensitivity Electrospray Ionization−Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Proteins
Citations Over TimeTop 1% of 2000 papers
Abstract
Methods are being developed for ultrasensitive protein characterization based upon electrospray ionization (ESI) with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The sensitivity of a FTICR mass spectrometer equipped with an ESI source depends on the overall ion transmission, which combines the probability of ionization, transmission efficiency, and ion trapping in the FTICR cell. Our developments implemented in a 3.5 tesla FTICR mass spectrometer include introduction and optimization of a newly designed electrodynamic ion funnel in the ESI interface, improving the ion beam characteristics in a quadrupole-electrostatic ion guide interface, and modification of the electrostatic ion guide. These developments provide a detection limit of approximately 30 zmol (approximately 18,000 molecules) for proteins with molecular weights ranging from 8 to 20 kDa.
Related Papers
- → Top-Down Analysis of Highly Post-Translationally Modified Peptides by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry(2014)19 cited
- → Rapid Analysis of Tryptically Digested Cerebrospinal Fluid Using Capillary Electrophoresis−Electrospray Ionization−Fourier Transform Ion Cyclotron Resonance−Mass Spectrometry(2002)63 cited
- → Efficient injection of low‐mass ions into high magnetic field Fourier transform ion cyclotron resonance mass spectrometers(2013)8 cited
- → MALDI Mass Spectrometry Instrumentation(2013)
- Comparison of bottom-up protein identification methods(2006)