Carboxylesterase and Cytochrome P450 Confer Metabolic Resistance Simultaneously to Azoxystrobin and Some Other Fungicides in Botrytis cinerea

Citations Over TimeTop 10% of 2024 papers

Abstract

Plant pathogens have frequently shown multidrug resistance (MDR) in the field, often linked to efflux and sometimes metabolism of fungicides. To investigate the potential role of metabolic resistance in B. cinerea strains showing MDR, the azoxystrobin-sensitive strain B05.10 and -resistant strain Bc242 were treated with azoxystrobin. The degradation half-life of azoxystrobin in Bc242 (9.63 days) was shorter than that in B05.10 (28.88 days). Azoxystrobin acid, identified as a metabolite, exhibited significantly lower inhibition rates on colony and conidia (9.34 and 11.98%, respectively) than azoxystrobin. Bc242 exhibited higher expression levels of 34 cytochrome P450s (P450s) and 11 carboxylesterase genes (CarEs) compared to B05.10 according to RNA-seq analysis. The expression of P450 genes Bcin_02g01260 and Bcin_12g06380, along with the CarEs Bcin_12g06360 in Saccharomyces cerevisiae, resulted in reduced sensitivity to various fungicides, including azoxystrobin, kresoxim-methyl, pyraclostrobin, trifloxystrobin, iprodione, and carbendazim. Thus, the mechanism of B. cinerea MDR is linked to metabolism mediated by the CarE and P450 genes.

Related Papers

• → Roˆle of antimicrobial substances (tuliposides) in tulips attacked by Botrytis spp.(1972)37 cited
• → Effect of Cultural Methods on the Occurrence of Grey Mould (Botrytis cinereaPers.) in Strawberries(2000)16 cited
• Development of a selective medium for the determination of the spore concentrations of Botrytis cinerea in the air.(2003)
• A Study on Selecting the Optimal Concentration of Sulfur Dioxide to Inhibit Botrytis cinerea Pers on Grapes(2013)
• Studies on Factors Affecting Botrytis Diseases on Certain Crop Plants(1953)