Ultra-efficient Antibacterial System Based on Photodynamic Therapy and CO Gas Therapy for Synergistic Antibacterial and Ablation Biofilms

Citations Over TimeTop 10% of 2020 papers

Abstract

In recent years, with the emergence of various kinds of drug-resistant bacteria, existing antibiotics have become inefficient in killing these bacteria, and the formation of biofilms has further weakened the therapeutic effect. More problematically, the massive use and abuse of antibiotics have caused severe side effects. Thus, the development of ultra-efficient and safe antibacterial systems is urgently needed. Herein, a photodynamic therapy (PDT)-driven CO-controlled delivery system (Ce6&CO@FADP) is developed for synergistic antibacterial and ablation biofilms. Ce6&CO@FADP is constructed using a fluorinated amphiphilic dendritic peptide (FADP) and physically loaded with Ce6 and CORM-401. After efficiently entering the bacteria, Ce6&CO@FADP can rapidly release CO intracellularly by the massive consumption of the H₂O₂ generated during the PDT process, without affecting the generation of singlet oxygen (1O₂). As such, the combination of CO and 1O₂ exerts notable synergistic antibacterial and biofilm ablation effects both in vitro and in vivo (including subcutaneous bacterial infection and biofilm catheter models) experiments. More importantly, all biosafety assessments suggest the good biocompatibility of Ce6&CO@FADP. Together, these results reveal that Ce6&CO@FADP is an efficient and safe antibacterial system, which has essential application prospects for the treatment of bacterial infections and ablation of biofilms in vivo.

Related Papers

• → Biofilms on instruments and environmental surfaces: Do they interfere with instrument reprocessing and surface disinfection? Review of the literature(2019)42 cited
• → In-vivo singlet oxygen dosimetry of clinical 5-aminolevulinic acid photodynamic therapy(2008)39 cited
• → Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors(2017)14 cited
• → Design of novel photosensitizers and controlled singlet oxygen generation for photodynamic therapy(2021)11 cited
• → Biofilms, Microbial(1999)