Facile Preparation of Core–Shell Magnetic Metal–Organic Framework Nanoparticles for the Selective Capture of Phosphopeptides

Citations Over TimeTop 10% of 2015 papers

Abstract

In regard to the phosphoproteome, highly specific and efficient capture of heteroideous kinds of phosphopeptides from intricate biological sample attaches great significance to comprehensive and in-depth phosphorylated proteomics research. However, until now, it has been a challenge. In this study, a new-fashioned porous immobilized metal ion affinity chromatography (IMAC) material was designed and fabricated to promote the selectivity and detection limit for phosphopeptides by covering a metal-organic frameworks (MOFs) shell onto Fe3O4 nanoparticles, taking advantage of layer-by-layer method (the synthesized nanoparticle denoted as Fe3O4@MIL-100 (Fe)). The thick layer renders the nanoparticles with perfect hydrophilic character, super large surface area, large immobilization of the Fe(3+) ions and the special porous structure. Specifically, the as-synthesized MOF-decorated magnetic nanoparticles own an ultra large surface area which is up to 168.66 m(2) g(-1) as well as two appropriate pore sizes of 1.93 and 3.91 nm with a narrow grain-size distribution and rapid separation under the magnetic circumstance. The unique features vested the synthesized nanoparticles an excellent ability for phosphopeptides enrichment with high selectivity for β-casein (molar ratio of β-casein/BSA, 1:500), large enrichment capacity (60 mg g(-1)), low detection limit (0.5 fmol), excellent phosphopeptides recovery (above 84.47%), fine size-exclusion of high molecular weight proteins, good reusability, and desirable batch-to-batch repeatability. Furthermore, encouraged by the experimental results, we successfully performed the as-prepared porous IMAC nanoparticle in the specific capture of phosphopeptides from the human serum (both the healthy and unhealthy) and nonfat milk, which proves itself to be a good candidate for the enrichment and detection of the low-abundant phosphopeptides from complicated biological samples.

Related Papers

• → Enhanced selective separation of fine particles from Cs-contaminated soil using magnetic nanoparticles(2020)11 cited
• → Selective separation of biomolecules from food waste by magnetic fishing technique - A review(2015)2 cited
• Progress on applications of magnetic nanoparticles in separation and detection(2012)
• Evaluation study on separation efficiency of E.coli O157：H7 using a new magnetic bioseparator with magnetic nanobeads(2015)
• → Modeling of the Bacteria attached with Magnetic Nanoparticles for Optimization of Magnetic Separation Process(2015)