Self-Assembled DNA Immunonanoflowers as Multivalent CpG Nanoagents
Citations Over TimeTop 10% of 2015 papers
Abstract
Synthetic unmethylated cytosine-guanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent immunostimulators by triggering the proliferation of these immune cells, which, in turn, secreted immunostimulatory cytokines, including tumor necrosis factor α, interleukin-6, and interleukin-10. These results demonstrate the ability of CpG NFs to induce cancer cell apoptosis and necrosis.
Related Papers
- → Response of nitric oxide production to CpG oligodeoxynucleotides in turkey and chicken peripheral blood monocytes(2006)26 cited
- → Dual Activity of Phosphorothioate CpG Oligodeoxynucleotides on HIV(2006)16 cited
- → A dual-amplification fluorescent sensing platform for ultrasensitive assay of nuclease and ATP based on rolling circle replication and exonuclease III-aided recycling(2015)7 cited
- → Requirements for Effective Inhibition of Immunostimulatory CpG Motifs by Neutralizing Motifs(2000)26 cited
- Delineation of CpG motifs for activating human immune cells(2001)