Increasing Enzyme Cascade Throughput by pH-Engineering the Microenvironment of Individual Enzymes
ACS Catalysis2017Vol. 7(3), pp. 2047–2051
Citations Over TimeTop 10% of 2017 papers
Abstract
The throughput of enzyme cascade reactions could be increased if the environmental conditions could be optimized for each enzyme in the cascade individually. Here, we describe the engineering of the microenvironment of one enzyme (Cytochrome C (abbreviated as Cyt C), which is active under acidic conditions), with respect to pH, so that it operates optimally together with a second enzyme (d-amino acid oxidase, which is active under alkaline conditions). Conjugation of Cyt C with a negatively charged polyelectrolyte lowers the local pH at the Cyt C active site and enables a 10-fold enhancement in cascade throughput.
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