Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence “Turn-On” Probe
ACS Chemical Biology2016Vol. 12(1), pp. 200–205
Citations Over TimeTop 10% of 2016 papers
Abstract
Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.
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