Extracellular Vesicles Mimetic Design of Membrane Chimeric Nanovesicles for dsRNA Delivery in Spray-Induced Gene Silencing for Crop Protection

Citations Over TimeTop 10% of 2024 papers

Abstract

Spray-induced gene silencing (SIGS) presents a promising RNA interference (RNAi)-based crop protection strategy against eukaryotic phytopathogens. However, the application of SIGS faces challenges, such as the limited uptake of dsRNA by certain pathogens and the instability of dsRNA in the environment. This study introduces innovative biomimetic nanovesicles, called extracellular vesicle (EV) mimetic chimeric nanovesicles (ECNs), assembled from tomato leaf cell membranes and cationic sterosomes via the freeze-thaw method. Similar to the function of EVs in nucleic acid transport between cells, ECNs serve as a hybrid nanosystem to overcome the challenge of delivering exogenous dsRNA in Phytophthora infestans. When applied to SIGS, the superiority of ECNs in crop protection becomes more apparent, including high loading and protection of dsRNA, improved biosafety, and efficient internalization into pathogen and plant cells, all of which significantly enhance the efficacy of RNAi in preventing early infection of P. infestans to susceptible tomato plants. This study demonstrates that ECNs are promising RNA delivery vehicles and will promote the use of SIGS-based RNA pesticides in sustainable agricultural production.

Related Papers

• → Enhancing RNAi by using concatemerized double‐stranded RNA(2018)40 cited
• → Influence of diverse storage conditions of double‐stranded RNAin vitro on the RNA interference efficiency in vivo insect Tribolium castaneum(2022)11 cited
• → Protection of Crops Against Insect Pests Using RNA Interference(2010)14 cited
• Feasibility, limitation and possible solutions of RNAi-based technology for insect pest control(2013)
• Delivery of dsRNA for RNAi in insects： an overview and future directions(2013)