Use of N-terminal modified poly(L-lysine)-antibody conjugate as a carrier for targeted gene delivery in mouse lung endothelial cells

Citations Over TimeTop 10% of 1992 papers

Abstract

A DNA targeted delivery and expression system has been designed based on an N-terminal modified poly(L-lysine) (NPLL)-antibody conjugate, which readily forms a complex with plasmid DNA. Monoclonal antibodies against the cell-surface thrombomodulin conjugated with NPLL were used for targeted delivery of foreign plasmid DNA to an antigen-expressing mouse lung endothelial cell line in vitro and to mouse lungs in vivo. In both cases significant amounts of DNA can be specifically bound to the target cells or tissues. Specific gene expression was observed in the treated mouse lung endothelial cells.

Related Papers

• → Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids(2017)89 cited
• → Studies of Reversible Conjugate Additions(2013)61 cited
• → Successful spread of mcr-1-bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids(2024)7 cited
• Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain.(1989)
• → Successful spread of mcr-1 -bearing IncX4 plasmids is associated with variant in replication protein of IncX4 plasmids(2023)