Transfection of Folate-Polylysine DNA Complexes: Evidence for Lysosomal Delivery
Citations Over Time
Abstract
We are utilizing the folate receptor for the intracellular delivery of DNA. In this study, a folate-poly-L-lysine (FPLL) conjugate was synthesized and equilibrated with plasmid DNA encoding the firefly luciferase gene. The FPLL-DNA complexes were added to KB cells treated with chloroquine. Luciferase activity of cells incubated with FPLL-DNA was 6-fold higher than of cells exposed to poly-L-lysine (PLL)-DNA. The addition of free folic acid competitively inhibited the enhancement of gene expression. Removal of chloroquine from the media significantly inhibited transfection efficiency of FPLL-DNA complexes. We conclude that FPLL-DNA complexes are delivered into KB cells via folate receptor-mediated endocytosis and likely follow a lysosomal pathway into the cytoplasm.
Related Papers
- → A Comparative Study of Users’ Microblogging Behavior on Sina Weibo and Twitter(2012)259 cited
- → Comparision of microblogging service between Sina Weibo and Twitter(2011)49 cited
- → News comments generation via mining microblogs(2012)15 cited
- → Summarizing the differences from microblogs(2012)2 cited
- → The Study on Factors Affecting the Attraction of Microblog – An Empirical Study Based on Sina Microblogging Site(2017)1 cited