Matrix Metalloproteinase-Assisted Triggered Release of Liposomal Contents

Citations Over TimeTop 13% of 2007 papers

Abstract

We offer a novel methodology for formulating liposomes by incorporating sequence-specific collagen-mimetic peptides such that they are specifically "uncorked" by a matrix metalloproteinase, MMP-9. By encapsulating carboxyfluorescein (as a self-quenching fluorescent dye), we demonstrate that the time-dependent release of the dye from liposomes is due to the specific enzymatic cleavage of the surface-exposed collagen-mimetic peptides. The specificity of such cleavage is attested by the fact that the liposomal "uncorking" and their content release occur only by MMP-9 and not by a general proteolytic enzyme, trypsin, despite the fact that the collagen mimetic peptides contain the trypsin cleavage site. The mechanistic details underlying the formulations of liposomes and their enzyme-selective "uncorking" and content release are discussed. Arguments are presented that such liposomes can be fine-tuned to serve as the drug delivery vehicles for the detection and treatment of various human diseases, which occur due to the overexpression of a variety of pathogenic matrix metalloproteinases.

Related Papers

• → Binding of Active (57 kDa) Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) to Tissue Inhibitor of Metalloproteinase (TIMP)-2 Regulates MT1-MMP Processing and Pro-MMP-2 Activation(2000)307 cited
• → Expanding the Activity of Tissue Inhibitors of Metalloproteinase (TIMP)-1 against Surface-Anchored Metalloproteinases by the Replacement of Its C-Terminal Domain: Implications for Anti-Cancer Effects(2015)15 cited
• → Transgenesis techniques : principles and protocols(2002)15 cited
• → Expression and activation of matrix metalloproteinase‐2 (MMP‐2) and its co‐localization with membrane‐type 1 matrix metalloproteinase (MT1‐MMP) correlate with melanoma progression(2000)7 cited
• Expression of Matrix Metalloproteinase (MMP) and Tissue Inhibitors of Metalloproteinase (TIMP) mRNA in Conjunctival Melanoma Cell Lines(2007)