Translocation Properties of Novel Cell Penetrating Transportan and Penetratin Analogues
Bioconjugate Chemistry2000Vol. 11(5), pp. 619–626
Citations Over TimeTop 11% of 2000 papers
Maria Lindgren, Xavier Gallet, Ursel Soomets, Mattias Hällbrink, Ebba Bråkenhielm, Margus Pooga, Robert Brasseur, Ülo Langel
Abstract
Novel analogues of the cell-penetrating peptides penetratin and transportan were synthesized. The distribution of the biotin-labeled peptides in Bowes melanoma cell line has been investigated by indirect fluorescence with fluorescein-streptavidin detection. The time course of uptake of (125)I-labeled transportan analogues has been characterized in the same cell line. Molecular modeling was used to analyze the penetration and the orientation of molecules in a simulated biological membrane. The results, both from molecular modeling and fluorescence studies, imply that penetratin and transportan do not enter the cells by related mechanisms and that they do not belong to the same family of translocating peptides.
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