Structural Characterization of PNA-DNA Duplexes by NMR. Evidence for DNA in a B-like Conformation
Biochemistry1994Vol. 33(33), pp. 9820–9825
Citations Over TimeTop 10% of 1994 papers
Mikael Leijon, Astrid Graeslund, Peter E. Nielsen, Ole Buchardt, Bengt Nordén, Søren M. Kristensen, Magdalena Eriksson
Abstract
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
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