Cloning and sequence determination of a complementary DNA related to human liver microsomal cytochrome P-450 S-mephenytoin 4-hydroxylase
Citations Over TimeTop 1% of 1987 papers
Abstract
A cDNA sequence related to the human cytochrome P-450 responsible for S-mephenytoin 4-hydroxylation (P-450MP) has been isolated from a human liver bacteriophage lambda gt11 library with antibodies specific for P-450MP. The total length of the cDNA is 2.5 kilobases (kb), of which there is a 1.6-kb EcoRI fragment coding for all but five amino acids corresponding to the N-terminus of the protein and including a small noncoding region at the 3' end. This 1.6-kb fragment has been sequenced and used as a probe to analyze human genomic DNA and liver RNA. The sequence shows extensive sequence similarity with that of rabbit liver cytochrome P-450 progesterone 21-hydroxylase [Tukey, R. H., Okino, S., Barnes, H., Griffin, K. J., & Johnson, E. F. (1985) J. Biol. Chem. 260, 13347-13354], and this cDNA, like the rabbit clone, appears to be part of a multigene family. At least two liver mRNA species, 2.2 kb and 3.5 kb, hybridize to the cDNA sequence. The cloning of this gene should aid in analyzing the molecular basis for the genetic polymorphism of S-mephenytoin 4-hydroxylation reported in humans.
Related Papers
- Role of CYP2C19 in stereoselective hydroxylation of mephobarbital by human liver microsomes.(2001)
- → Interactions between solubilized cytochrome P-450 and hepatic microsomes.(1977)60 cited
- → 25-hydroxylation of vitamin D3 in rat liver: Roles of mitochondrial and microsomal cytochrome P-450(1987)21 cited
- → N-METHYLCARBAZOLE METABOLISM: A PROBE FOR CHARACTERIZING THE MULTIPLE FORMS OF CYTOCHROME P-450(1980)
- [Effect of the lipophilic spin-labeled inhibitor of cytochrome P-450 on the activity of the microsomal system].(1987)