A Putative Fe 2+ -Bound Persulfenate Intermediate in Cysteine Dioxygenase
Biochemistry2008Vol. 47(44), pp. 11390–11392
Citations Over TimeTop 13% of 2008 papers
C.R. Simmons, Kalyanaraman Krishnamoorthy, Spencer L. Granett, David J. Schuller, John E. Dominy, Tadhg P. Begley, Martha H. Stipanuk, P. Andrew Karplus
Abstract
The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediate trapped in its active site pocket. The complex raises the possibility that, distinct from known dioxygenases and proposed CDO mechanisms, the Fe-proximal oxygen atom may be involved in the primary oxidation event yielding a unique three-membered Fe-S-O cyclic intermediate. A nonpolar environment of the distal oxygen would facilitate isomerization of the persulfenate to the sulfinate product.
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