Putative Human Blue-Light Photoreceptors hCRY1 and hCRY2 Are Flavoproteins
Citations Over TimeTop 1% of 1996 papers
Abstract
Recently, a human cDNA clone with high sequence homology to the photolyase/blue-light photoreceptor family was identified. The putative protein encoded by this gene exhibited a strikingly high (48% identity) degree of homology to the Drosophila melanogaster (6-4) photolyase [Todo et al. (1996) Science 272, 109-112]. We have now identified a second human gene whose amino acid sequence displays 73% identity to the first one and have named the two genes CRY1 and CRY2, respectively. The corresponding proteins hCRY1 and hCRY2 were purified and characterized as maltose-binding fusion proteins. Similar to other members of the photolyase/blue-light photoreceptor family, both proteins were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct. We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans.
Related Papers
- → A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity(2006)293 cited
- → Photolyase and Cryptochrome Blue-Light Photoreceptors(2004)85 cited
- → A Study on Yeast Using the Photoreactivation Process to Repair the Pyrimidine Dimer Mutations(2023)1 cited
- → (6-4) photolyase: light-dependent repair of DNA damage.(1998)19 cited
- → Faculty Opinions recommendation of Enhanced repair of cyclobutane pyrimidine dimers and improved UV resistance in photolyase transgenic mice.(2002)