Optimization of Chitin Extraction from Shrimp Shells

Citations Over TimeTop 10% of 2002 papers

Abstract

The aim of this paper is to define optimal conditions for the extraction of chitin from shrimp shells. The kinetics of both demineralization and deproteinization with, in the latter case, the role of temperature are studied. The characterization of the residual calcium and protein contents, the molecular weights, and degrees of acetylation (DA) allows us to propose the optimal conditions as follows. The demineralization is completely achieved within 15 min at ambient temperature in an excess of HCl 0.25 M (with a solid-to-liquid ratio of about 1/40 (w/v)). The deproteinization is conveniently obtained in NaOH 1 M within 24 h at a temperature close to 70 degrees C with no incidence on the molecular weight or the DA. In these conditions, the residual content of calcium in chitin is below 0.01%, and the DA is almost 95%.

Related Papers

• → Rate and Mechanism of Enamel Demineralization in situ(1992)34 cited
• → Effect of Loading and pH on the Subsurface Demineralization of Dentin Beams(2006)16 cited
• → Rapid demineralization in acidic buffers(1979)30 cited
• Recent progress in penaeid shrimp culture(1977)
• → Demineralization of skimmed milk by electrodialysis with ion exchange and acetylcellulose membrane.(1987)2 cited