Characterization of Shrimp Shell Deproteinization
Citations Over TimeTop 20% of 2003 papers
Abstract
The aim of this paper was to contribute to the interpretation of the mechanism of shrimp shell deproteinization. We used amino acid analysis to quantify the amount of proteins remaining in chitin. NaOH 1 M was added to a demineralized shrimp shell powder with a solution-to-solid ratio of 15 mL/g at ambient temperature. Because of the limited precision of the technique, after 24 h the protein content measured by elemental analysis had to be considered as negligible. However, with the use of amino acid analysis, it was still possible to determine with precision this content down to 0.25%. We also showed that among the peptides remaining linked to chitin after deproteinization, acidic amino acids were always in proportion higher than alkaline ones, but the balance between the two kinds of residues increased in favor of the latter with time. The kinetic study of the deproteinization clearly revealed a three-step mechanism with very different rate constants. The variation of these constants with temperature was used to calculate the energies of activation and the frequency factors of collision, thus allowing us to propose a new interpretation of the mechanism of deproteinization.
Related Papers
- → Chitin derived from Daphnia similis and its derivate, chitosan, promote growth performance of Penaeus vannamei(2020)27 cited
- → Effect of Shrimp Processing Procedures on the Quality and Quantity of Extracted Chitin from the Shells of Northern Shrimp Pandalus borealis(2003)16 cited
- Study on the Preparation of Chitin with EDTA Decalcify Method(2006)
- Optimization of Chitin Extraction from Shrimp and Crab Shell in Chaoshan Area(2015)
- Preparation of chitin and chitosan from different kinds of shrimp shell(2004)