In Vitro Reconstitution of Fibrillar Collagen Type I Assemblies at Reactive Polymer Surfaces
Citations Over TimeTop 17% of 2004 papers
Abstract
The reconstitution of fibrillar collagen and its assemblies with heparin and hyaluronic acid was studied in vitro. Fibril formation kinetics were analyzed by turbidity and depletion measurements in solutions containing varied concentrations of collagen and glycosaminoglycans. Fibril-forming collagen solutions were further applied for the coating of planar substrates which had been modified with alternating maleic anhydride copolymer films before. The immobilized collagen assemblies were characterized with respect to the deposited amount of protein using ellipsometry and acidic hydrolysis/HPLC-based amino acid analysis, respectively. AFM, SEM, and cLSM were utilized to gain information on structural features and patterns formed by surface-attached fibrils depending on the initial solution concentrations of collagen. The results revealed that the addition of heparin and hyaluronic acid affected both the fibril dimensions and the meshwork characteristics of the surface-bound fibrils.
Related Papers
- Hyaluronic Acid Production and its Applications A Review(2013)
- → Characterization of epidermal glycosaminoglycans synthesized in organ culture(1981)39 cited
- → Incorporation of [3H]glucosamine into glycosaminoglycans in different cell culture conditions by rabbit aortic smooth muscle cells(1981)4 cited
- STUDY ON COPOLYMERIZATION OF D_3~F, D_4(2002)
- Application of hyaluronic acid to oral medicine(2013)