Patterned Protein Films on Poly(lipid) Bilayers by Microcontact Printing

Citations Over TimeTop 10% of 2006 papers

Abstract

The use of polymerized lipid bilayers as substrates for microcontact printing (muCP) of protein films was investigated. We have previously shown that vesicle fusion of bis-SorbPC, a dienoate lipid, on glass and silica substrates, followed by redox-initiated radical polymerization, produces a planar supported lipid bilayer (PSLB) that is ultrastable(1a) [Ross, E. E.; Rozanski, L. J.; Spratt, T.; Liu, S.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 1752] and highly resistant to nonspecific adsorption of dissolved proteins [Ross, E. E.; Spratt, T.; Liu, S.; Rozanski, L. J.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 1766].(1b) Here we demonstrate that muCP of bovine serum albumin (BSA) onto a dried poly(bis-SorbPC) PSLB from a poly(dimethylsiloxane) (PDMS) stamp produces a layer of strongly adsorbed protein, comparable in surface coverage to films printed on glass surfaces. Immobilization of proteins on poly(PSLB)s has potential applications in biosensing, and this work shows that direct muCP of proteins is a technically simple approach to create immobilized monolayers, as well as multilayers of different proteins.

Related Papers

• → Interaction of an Antimicrobial Peptide with a Model Lipid Bilayer Using Molecular Dynamics Simulation(2009)28 cited
• → Effect of the structural difference between Bax-α5 and Bcl-xL-α5 on their interactions with lipid bilayers(2013)6 cited
• To Study the Height and Topography of Lipid Bilayer by AFM(2007)
• → The Role of Bilayer Edges in Supported Lipid Bilayer Formation at Low Lipid Concentrations(2009)
• → Interdependence of cholesterol distribution and conformational order in lipid bilayers(2023)