Fluorescent Dye Cocktail for Multiplex Drug-Site Mapping on Human Serum Albumin
ACS Combinatorial Science2013Vol. 15(9), pp. 452–457
Citations Over TimeTop 13% of 2013 papers
Abstract
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
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