Are Toxicological Responses in Laboratory (Inbred) Zebrafish Representative of Those in Outbred (Wild) Populations? − A Case Study with an Endocrine Disrupting Chemical

Citations Over TimeTop 10% of 2011 papers

Abstract

Laboratory animals tend to be more inbred and less genetically diverse than wild populations, and thus may differ in their susceptibility to chemical stressors. We tested this hypothesis by comparing the responses of related inbred (theoretical inbreeding F(IT) = n + 0.25) and outbred (F(IT) = n) zebrafish (Danio rerio) WIK/Wild family lines to an endocrine disrupting chemical, clotrimazole. Exposure of inbred and outbred zebrafish to 2.9 μg clotrimazole/L had no effect on survival, growth, or gonadal development. Exposure of both lines to 43.7 μg clotrimazole/L led to male-biased sex ratios compared with controls (87% versus 55% and 92% vs 64%, for inbred and outbred males, respectively), advanced germ cell development, and reduced plasma 11-ketotestosterone concentrations in males. However, outbred males (but not inbred males) developed testis that were more than twice the weight of controls, which corresponded with a proliferation of Leydig cells and maintenance of the expression (rather than down-regulation occurring in inbreds) of gonadal aromatase (cyp19a1a) and insulin-like growth factor (igf1). Our results illustrate that the effects of an endocrine disrupting chemical (clotrimazole) on some end points (here testis development) can differ between inbred and outbred zebrafish. This highlights the need for reporting pedigree/genetic information and consistency in the responses of laboratory animals (e.g., by using model compounds as positive controls).

Related Papers

• → Copper caused reproductive endocrine disruption in zebrafish (Danio rerio)(2019)75 cited
• → Genomic Analysis Reveals Specific Patterns of Homozygosity and Heterozygosity in Inbred Pigs(2019)13 cited
• → Two common mild analgesics have no effect on general endocrine mediated endpoints in zebrafish (Danio rerio)(2017)5 cited
• Advances of studies on Effects of Endocrine Disrupting Chemicals on Growth and Development of Zebrafish(Danio rerio)(2008)
• Regulation of the endocrine stress response and the modulating effects of sex steroids in zebrafish (Danio rerio)(2010)