Species Differences Take Shape at Nanoparticles: Protein Corona Made of the Native Repertoire Assists Cellular Interaction

Citations Over TimeTop 10% of 2013 papers

Abstract

Cells recognize the biomolecular corona around a nanoparticle, but the biological identity of the complex may be considerably different among various species. This study explores the importance of protein corona composition for nanoparticle recognition by coelomocytes of the earthworm Eisenia fetida using E. fetida coelomic proteins (EfCP) as a native repertoire and fetal bovine serum (FBS) as a non-native reference. We have profiled proteins forming the long-lived corona around silver nanoparticles (75 nm OECD reference materials) and compared the responses of coelomocytes to protein coronas preformed of EfCP or FBS. We find that over time silver nanoparticles can competitively acquire a biological identity native to the cells in situ even in non-native media, and significantly greater cellular accumulation of the nanoparticles was observed with corona complexes preformed of EfCP (p < 0.05). An EfCP-nanoparticle mimicry made with a recombinant protein, lysenin, revealed its critical contribution in the observed cell-nanoparticle response. This confirms the determinant role of the recognizable biological identity during invertebrate in vitro testing of nanoparticles. Our finding shows a case of species-specific formation of biomolecular coronas, and this suggests that the use of representative species may need careful consideration in assessing the risks associated with nanoparticles.

Related Papers

• → Element repertoire: change and development with age in Whitethroat Sylvia communis song(2009)16 cited
• BAĞLAMADA ENSTRÜMANTAL REPERTUVARIN ÇEŞİTLİ DEĞİŞKENLER AÇISINDAN İNCELENMESİ(2020)
• → The Results of Bandstand Dynamics(2013)
• → Challenges and New Insights in the Critical Edition of the Persianate Repertoire in Ottoman Music(2023)
• → МЕТОДИКА ПОДБОРА РЕПЕРТУАРА(2023)