A Bioaccumulative Cyclometalated Platinum(II) Complex with Two-Photon-Induced Emission for Live Cell Imaging
Citations Over TimeTop 10% of 2009 papers
Abstract
The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C(phenyl), N(pyridyl), and N(pyrazolyl) donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.
Related Papers
- → Large-Scale Screening Assay to Measure Epidermal Growth Factor Internalization(2000)12 cited
- → Role of carboxyl tail of the rat angiotensin II type 1A receptor in agonist-induced internalization of the receptor(1994)32 cited
- Antibody transport and internalization into tumours.(1990)
- → The Internalization and Therapeutic Activity of Antibody Drug Conjugates(2023)