Intein-Mediated Biotinylation of Proteins and Its Application in a Protein Microarray
Journal of the American Chemical Society2002Vol. 124(30), pp. 8768–8769
Citations Over TimeTop 10% of 2002 papers
Abstract
We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays.
Related Papers
- → Tamavidins – novel avidin‐like biotin‐binding proteins from the Tamogitake mushroom(2009)64 cited
- → Interference of sugars with the binding of biotin to streptavidin and avidin(1997)19 cited
- → Hormonal Properties of Avidin-Biotinylinsulin and Avidin-Biotinylcorticotropin Complexes(1981)9 cited
- → Colloidal Gold/Streptavidin Methods(1994)
- → Colloidal Gold/Streptavidin Methods(2003)