Writing with DNA and Protein Using a Nanopipet for Controlled Delivery
Journal of the American Chemical Society2002Vol. 124(30), pp. 8810–8811
Citations Over TimeTop 1% of 2002 papers
Andreas Bruckbauer, Liming Ying, Alison M. Rothery, Dejian Zhou, Andrew Shevchuk, Chris Abell, Yuri E. Korchev, David Klenerman
Abstract
We present a new, general method for the controlled deposition of biological molecules on surfaces, based on a nanopipet operating in ionic solution. The potential applied to the pipet tip controls the flux of biological molecules from the pipet, allowing fine control of the delivery rate. We used the ion current to control the distance of the pipet from the surface of a glass slide and deposited the fluorescently labeled DNA or protein G at a defined location onto the surface. Features of 830 nm size were obtained by depositing the biotinylated DNA onto a streptavidin surface; 1.3 mum size spots were obtained by depositing protein G onto a positively charged glass surface.
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