Label-Free DNA Hybridization Probe Based on a Conducting Polymer
Citations Over TimeTop 10% of 2002 papers
Abstract
A new approach to the realization of the electrochemical DNA hybridization probe is described. It is based on the exchange of chloride ion between the polypyrrole layer and the buffer. The shape of the cyclic voltammogram is modulated by the negative charge density at this interface, resulting from the immobilized target DNA. The negative charge density increases when the complementary DNA hybridizes with the probe DNA. The hybridization event can be clearly seen in the cyclic voltammogram before and after the addition of the probe DNA. The immobilization is accomplished via the Mg2+ bridging complex between phosphonic acid groups of the poly[2,5-dithienyl-(N-3-phosphorylpropyl)pyrrole] grafted at the polypyrrole surface and the phosphate groups of the target DNA.
Related Papers
- → DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA.(2000)175 cited
- → Effect of reagent concentrations used to synthesize polypyrrole on the chemical characteristics and optical and electronic properties of the resulting polymer(1992)215 cited
- → DNA probe specific for Legionella pneumophila(1985)53 cited
- → DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens(1987)14 cited
- Electrochemical detection of DNA hybridization using methylene blue as an electrochemical hybridization indicator(2007)