Structure of Antibacterial Peptide Microcin J25: A 21-Residue Lariat Protoknot
Journal of the American Chemical Society2003Vol. 125(41), pp. 12382–12383
Citations Over TimeTop 10% of 2003 papers
Marvin J. Bayro, Jayanta Mukhopadhyay, G.V.T. Swapna, Janet Huang, Li-Chung Ma, Elena V. Sineva, Philip E. Dawson, G.T. Montelione, Richard H. Ebright
Abstract
The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
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