Reversible Modulation of Quantum Dot Photoluminescence Using a Protein- Bound Photochromic Fluorescence Resonance Energy Transfer Acceptor
Citations Over TimeTop 10% of 2003 papers
Abstract
Multiple copies ( approximately 20) of Escherichia coli maltose binding protein (MBP) were coordinated to luminescent semiconductor quantum dots (QDs) via a C-terminal oligohistidine segment. The MBP was labeled with a sulfo-N-hydroxysuccinimide-activated photochromic BIPS molecule (1',3-dihydro-1'-(2-carboxyethyl)-3,3-dimethyl-6-nitrospiro[2H-1-benzopyran-2,2'-(2H)-indoline]) at two different dye-to-MBP ratios; D/P = 1 and 5. The ability of MBP-BIPS to modulate QD photoluminescence was tested by switching BIPS from the colorless spiropyran (SP) to the colored merocyanine (MC) using white light (>500 nm) or UV light ( approximately 365 nm), respectively. QDs surrounded by MBP-BIPS with D/P = 1 were quenched on average approximately 25% with consecutive repeated switches, while QDs surrounded by MBP-BIPS with D/P = 5 were quenched approximately 60%. This result suggests a possible use of BIPS-labeled proteins in QD-based nanostructures as part of a threshold switch or other biosensing device.
Related Papers
- → Synthesis, Photochromic Properties, and Crystal Structures of Salts Containing a Pyridinium-Fused Spiropyran: Positive and Negative Photochromism in the Solution and Solid State(2020)23 cited
- → Photo-regenerable surface with potential for optical sensing(2006)80 cited
- → Efficient Negative Photochromism by the Photoinduced Migration of Photochromic Merocyanine/Spiropyran in the Solid State(2021)26 cited
- Synthesis and photochromic behavior of spiropyran dyes(2005)
- Recent Advances of Indoline Spiropyran Photochromic Compounds(2004)