Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level
Journal of the American Chemical Society2004Vol. 126(13), pp. 4240–4244
Citations Over TimeTop 1% of 2004 papers
Kim Doré, Sébastien Dubus, Hoang‐Anh Ho, Isabelle Lévesque, Maryse Brunette, Geneviève Corbeil, Maurice Boissinot, Guy Boivin, Michel G. Bergeron, Denis Boudreau, Mario Leclerc
Abstract
We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.
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