Streptavidin as a Macroinitiator for Polymerization: In Situ Protein−Polymer Conjugate Formation
Citations Over TimeTop 1% of 2005 papers
Abstract
This communication reports the first example of polymerization initiated from specific domains on proteins. Streptavidin was coupled with a biotinylated initiator for atom transfer radical polymerization (ATRP) and exposed to an aqueous solution of CuBr/2,2'-bipyridine and monomer. N-Isopropylacrylamide (NIPAAm) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) were readily initiated by the modified streptavidin and polymerized from the protein at room temperature. Formation of streptavidin-polymer conjugates was confirmed by size exclusion chromatography (SEC) and gel electrophoresis. Polymer identity and biotinylation was verified using 1H NMR spectroscopy, gel permeation chromatography (GPC), and surface plasmon resonance (SPR) after dissociation of the biotin-streptavidin complex. This general approach is likely to be extended to other proteins and monomers and promises to enable easy synthesis and purification of a variety of polymer-protein conjugates.
Related Papers
- → Purification of biotinylated proteins on streptavidin resin: A protocol for quantitative elution(2004)142 cited
- → Variability in Streptavidin–Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data(2020)15 cited
- → Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins(2008)16 cited
- → Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction(2009)14 cited
- → Improving tumour targeting and decreasing normal tissue uptake by optimizing the stoichiometry of a two-step biotinylated-antibody/streptavidin-based targeting strategy: Studies in a nude mouse xenograft model(1998)8 cited