A Phage Display System with Unnatural Amino Acids
Journal of the American Chemical Society2004Vol. 126(49), pp. 15962–15963
Citations Over TimeTop 10% of 2004 papers
Abstract
We report a general method to display peptide-containing unnatural amino acids on filamentous M13 phage. Five distinct unnatural amino acids were site-specifically incorporated at the N-terminal of the M13 phage minor coat protein pIII. Phages that contain p-azidophenylalanine can undergo a highly specific azide-alkyne [3 + 2] cycloaddition reaction with an alkyne-derivatized fluorophore. The generalization of phage display to include unnatural amino acids should significantly increase the scope of phage display technology.
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