Direct Photochemical Patterning and Refunctionalization of Supported Phospholipid Bilayers
Citations Over TimeTop 11% of 2004 papers
Abstract
A wet photolithographic route for micropatterning fluid phospholipid bilayers is demonstrated in which spatially directed illumination by short-wavelength ultraviolet radiation results in highly localized photochemical degradation of the exposed lipids. Using this method, we can directly engineer patterns of hydrophilic voids within a fluid membrane as well as isolated membrane corrals over large substrate areas. We show that the lipid-free regions can be refilled by the same or other lipids and lipid mixtures which establish contiguity with the existing membrane, thereby providing a synthetic means for manipulating membrane compositions, engineering metastable membrane microdomains, probing 2D lipid-lipid mixing, and designing membrane-embedded arrays of soluble proteins. Following this route, new constructs can be envisaged for high-throughput membrane proteomic, biosensor array, and spatially directed, aqueous-phase material synthesis.
Related Papers
- → Micropatterning Strategies to Engineer Controlled Cell and Tissue Architecture in Vitro(2015)92 cited
- → High-efficacy subcellular micropatterning of proteins using fibrinogen anchors(2020)23 cited
- → Microcontact Peeling as a New Method for Cell Micropatterning(2014)14 cited
- → J0270102 Analysis of the Surface Elemental Compositions and Hydrophilicity for Development of New Cell Micropatterning(2014)
- → Fibrinogen anchors for micropatterning of active proteins and subcellular receptor relocalisation(2020)