NMR Assignments for a Helical 40 kDa Membrane Protein
Journal of the American Chemical Society2004Vol. 126(16), pp. 5048–5049
Citations Over TimeTop 10% of 2004 papers
Abstract
Backbone nuclear magnetic resonance (NMR) assignments were achieved for diacylglycerol kinase (DAGK) in detergent micelles. DAGK is a homotrimeric integral membrane protein comprised of 121 residue subunits, each having three transmembrane segments. Assignments were made using TROSY-based pulse sequences. DAGK was found to be an almost exclusively helical protein. This work points to the feasibility of both solving the structure of DAGK using solution NMR methods and using NMR as a primary tool in structural studies of other helical integral membrane proteins of similar size and complexity.
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