Phosphorescent Oxygen Sensor with Dendritic Protection and Two-Photon Absorbing Antenna
Citations Over TimeTop 1% of 2005 papers
Abstract
Imaging oxygen in 3D with submicron spatial resolution can be made possible by combining phosphorescence quenching technique with multiphoton laser scanning microscopy. Because Pt and Pd porphyrin-based phosphorescent dyes, traditionally used as phosphors in biological oxygen measurements, exhibit extremely low two-photon absorption (2PA) cross-sections, we designed a nanosensor for oxygen, in which a 2P absorbing antenna is coupled to a metalloporphyrin core via intramolecular energy transfer (ET) with the purpose of amplifying the 2PA induced phosphorescence of the metalloporphyrin. The central component of the device is a polyfunctionalized Pt porphyrin, whose triplet state emission at ambient temperatures is strong, occurs in the near infrared and is sensitive to O2. The 2PA chromophores are chosen in such a way that their absorption is maximal in the near infrared (NIR) window of tissue (e.g., 700-900 nm), while their fluorescence is overlapped with the absorption band(s) of the core metalloporphyrin, ensuring an efficient antenna-core resonance ET. The metalloporphyrin-antenna construct is embedded inside the protecting dendritic jacket, which isolates the core from interactions with biological macromolecules, controls diffusion of oxygen and makes the entire sensor water-soluble. Several Pt porphyrin-coumarin based sensors were synthesized and their photophyics studied to evaluate the proposed design.
Related Papers
- → Aggregation induced phosphorescence of metal complexes: From principles to applications(2017)208 cited
- → Solid-state oxygen sensor using sputtered LaF3 film(1989)78 cited
- → On the nature of tyrosine phosphorescence from proteins(1966)19 cited
- → [11] Application of phosphorescence to the study of proteins(1978)7 cited
- Assessment of Oxygen Concentration Unit from Measured Oxygen Activity by Oxygen Sensor Based on Metallurgical Thermodynamics(2011)