Single-Walled Carbon Nanotube Biosensors Using Aptamers as Molecular Recognition Elements
Journal of the American Chemical Society2005Vol. 127(34), pp. 11906–11907
Citations Over TimeTop 1% of 2005 papers
Hye‐Mi So, Keehoon Won, Yong Hwan Kim, Byoung-Kye Kim, Beyong Hwan Ryu, Pil Sun Na, Hyojin Kim, Jeong-O Lee
Abstract
We report the real-time detection of protein using SWNT-FET-based biosensors comprising DNA aptamers as molecular recognition elements. Anti-thrombin aptamers that are highly specific to serine protein thrombin were immobilized on the sidewall of a SWNT-FET using CDI-Tween linking molecules. The binding of thrombin aptamers to SWNT-FETs causes a rightward shift of the threshold gate voltages, presumably due to the negatively charged backbone of the DNA aptamers. While the addition of thrombin solution causes an abrupt decrease in the conductance of the thrombin aptamer immobilized SWNT-FET, no noticeable change was observed with elastase.
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