Quantum Dot-Based Multiplexed Fluorescence Resonance Energy Transfer

Citations Over TimeTop 10% of 2005 papers

Abstract

We demonstrate the use of luminescent quantum dots (QDs) conjugated to dye-labeled protein acceptors for nonradiative energy transfer in a multiplexed format. Two configurations were explored: (1) a single color QD interacting with multiple distinct acceptors and (2) multiple donor populations interacting with one type of acceptor. In both cases, we showed that simultaneous energy transfer between donors and proximal acceptors can be measured. However, data analysis was simpler for the configuration where multiple QD donors are used in conjunction with one acceptor. Steady-state fluorescence results were corroborated by time-resolved measurements where selective shortening of QD lifetime was measured only for populations that were selectively engaged in nonradiative energy transfer.

Related Papers

• → [13] Fluorescence resonance energy transfer(1995)516 cited
• → Fluorescence Resonance Energy Transfer-Based Stoichiometry in Living Cells(2002)357 cited
• → Global analysis of Förster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting the rise time of acceptor fluorescence(2010)84 cited
• → Effect of plasmonic nanostructures and nanofilms on fluorescence resonance energy transfer(2009)29 cited
• → Fluorescence Lifetime-based Multiplexing of Near-Infrared Förster Resonance Energy Transfer Pairs(2016)